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Download free software - powerful deconvolution software in the BiaQIm image processing suite (32 bit and 64 bit multi-core enabled for PC). Learn about deconvolution - a restoration process useful in astronomy, microscopy, photography, forensics, spectroscopy and other areas. The same is true for the lower end. The experimenter should stop image acquisition just beneath the optimal focus. With an optimal setup and image acquisition, the rest of the work is delegated to a computer. By using different algorithms, deconvolution starts with the help of a suitable PSF to recalculate the real properties of the specimen.

Top 4 Download periodically updates software information of deconvolution full versions from the publishers, but some information may be slightly out-of-date.

Send comments to wsr nih.gov. Disclaimer nih.gov. Deconvolution is an advanced and open source image deblur software, which can unblur photos that are out of focus or shaky. It has a number of optimization methods to fix blurry pictures. Image deconvolution (sometimes known as image deblurring) is the process of reconstructing or estimating the true image from the degraded one. Image deblurring algorithms can be classified into two classes: spectral filtering methods and iterative methods. The deconvolution is an image-processing technique that restores the effective specimen representation for a 3D microscopy images. Various software packages for deconvolution are available, both commercial ones and open-source ones. They are computationally extensive requiring high-end processors and huge memory capacities. Because the source and distribution of common noise in digital mages is so well understood, it can be easily removed by application of the appropriate image filters, which are usually included in most deconvolution software packages as an optional 'pre-processing' routine.

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In mathematics, deconvolution is an algorithm-based process used to reverse the effects of convolution on recorded data.^[1] The concept of deconvolution is widely used in the techniques of signal processing and image processing. Because these techniques are in turn widely used in many scientific and engineering disciplines, deconvolution finds many applications.

In general, the objective of deconvolution is to find the solution of a convolution equation of the form:

${\displaystyle f\ast g=h}$

Usually, h is some recorded signal, and f is some signal that we wish to recover, but has been convolved with some other signal g before we recorded it. The function g might represent the transfer function of an instrument or a driving force that was applied to a physical system. If we know g, or at least know the form of g, then we can perform deterministic deconvolution. However, if we do not know g in advance, then we need to estimate it. This is most often done using methods of statisticalestimation.

In physical measurements, the situation is usually closer to

${\displaystyle (f\ast g)+\epsilon =h}$

In this case ε is noise that has entered our recorded signal. If we assume that a noisy signal or image is noiseless when we try to make a statistical estimate of g, our estimate will be incorrect. In turn, our estimate of ƒ will also be incorrect. The lower the signal-to-noise ratio, the worse our estimate of the deconvolved signal will be. That is the reason why inverse filtering the signal is usually not a good solution. However, if we have at least some knowledge of the type of noise in the data (for example, white noise), we may be able to improve the estimate of ƒ through techniques such as Wiener deconvolution.

Deconvolution is usually performed by computing the Fourier Transform of the recorded signal h and the transfer functiong, apply deconvolution in the Frequency domain, which in the case of absence of noise is merely:

${\displaystyle F=H/G}$

F, G, and H being the Fourier Transforms of f, g, and h respectively. Finally inverse Fourier TransformF to find the estimated deconvolved signal f.

The foundations for deconvolution and time-series analysis were largely laid by Norbert Wiener of the Massachusetts Institute of Technology in his book Extrapolation, Interpolation, and Smoothing of Stationary Time Series (1949).^[2] The book was based on work Wiener had done during World War II but that had been classified at the time. Some of the early attempts to apply these theories were in the fields of weather forecasting and economics.

• 1Applications

Applications[edit]

Seismology[edit]

The concept of deconvolution had an early application in reflection seismology. In 1950, Enders Robinson was a graduate student at MIT. He worked with others at MIT, such as Norbert Wiener, Norman Levinson, and economist Paul Samuelson, to develop the 'convolutional model' of a reflection seismogram. This model assumes that the recorded seismogram s(t) is the convolution of an Earth-reflectivity function e(t) and a seismicwaveletw(t) from a point source, where t represents recording time. Thus, our convolution equation is

${\displaystyle s(t)=(e\ast w)(t).}$

The seismologist is interested in e, which contains information about the Earth's structure. By the convolution theorem, this equation may be Fourier transformed to

${\displaystyle S(\omega )=E(\omega )W(\omega )}$

in the frequency domain. By assuming that the reflectivity is white, we can assume that the power spectrum of the reflectivity is constant, and that the power spectrum of the seismogram is the spectrum of the wavelet multiplied by that constant. Thus,

${\displaystyle S(\omega )\approx kW(\omega ).}$

If we assume that the wavelet is minimum phase, we can recover it by calculating the minimum phase equivalent of the power spectrum we just found. The reflectivity may be recovered by designing and applying a Wiener filter that shapes the estimated wavelet to a Dirac delta function (i.e., a spike). The result may be seen as a series of scaled, shifted delta functions (although this is not mathematically rigorous):

${\displaystyle e(t)=\sum _{i=1}^N{r}_i\delta (t-{\tau }_i)}$,

where N is the number of reflection events, τ_iτ_i are the reflection times of each event, and r_i are the reflection coefficients.

In practice, since we are dealing with noisy, finite bandwidth, finite length, discretely sampled datasets, the above procedure only yields an approximation of the filter required to deconvolve the data. However, by formulating the problem as the solution of a Toeplitz matrix and using Levinson recursion, we can relatively quickly estimate a filter with the smallest mean squared error possible. We can also do deconvolution directly in the frequency domain and get similar results. The technique is closely related to linear prediction.

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Optics and other imaging[edit]

In optics and imaging, the term 'deconvolution' is specifically used to refer to the process of reversing the optical distortion that takes place in an optical microscope, electron microscope, telescope, or other imaging instrument, thus creating clearer images. It is usually done in the digital domain by a softwarealgorithm, as part of a suite of microscope image processing techniques. Deconvolution is also practical to sharpen images that suffer from fast motion or jiggles during capturing. Early Hubble Space Telescope images were distorted by a flawed mirror and were sharpened by deconvolution.

The usual method is to assume that the optical path through the instrument is optically perfect, convolved with a point spread function (PSF), that is, a mathematical function that describes the distortion in terms of the pathway a theoretical point source of light (or other waves) takes through the instrument.^[3] Usually, such a point source contributes a small area of fuzziness to the final image. If this function can be determined, it is then a matter of computing its inverse or complementary function, and convolving the acquired image with that. The result is the original, undistorted image.

In practice, finding the true PSF is impossible, and usually an approximation of it is used, theoretically calculated^[4] or based on some experimental estimation by using known probes. Real optics may also have different PSFs at different focal and spatial locations, and the PSF may be non-linear. The accuracy of the approximation of the PSF will dictate the final result. Different algorithms can be employed to give better results, at the price of being more computationally intensive. Since the original convolution discards data, some algorithms use additional data acquired at nearby focal points to make up some of the lost information. Regularization in iterative algorithms (as in expectation-maximization algorithms) can be applied to avoid unrealistic solutions.

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When the PSF is unknown, it may be possible to deduce it by systematically trying different possible PSFs and assessing whether the image has improved. This procedure is called blind deconvolution.^[3] Blind deconvolution is a well-established image restoration technique in astronomy, where the point nature of the objects photographed exposes the PSF thus making it more feasible. It is also used in fluorescence microscopy for image restoration, and in fluorescence spectral imaging for spectral separation of multiple unknown fluorophores. The most common iterative algorithm for the purpose is the Richardson–Lucy deconvolution algorithm; the Wiener deconvolution (and approximations) are the most common non-iterative algorithms.

For some specific imaging systems such as laser pulsed terahertz systems, PSF can be modeled mathematically.^[6] As a result, as shown in the figure, deconvolution of the modeled PSF and the terahertz image can give a higher resolution representation of the terahertz image.

Radio astronomy[edit]

When performing image synthesis in radio interferometry, a specific kind of radio astronomy, one step consists of deconvolving the produced image with the 'dirty beam', which is a different name for the point spread function. A commonly used method is the CLEAN algorithm.

Fourier transform aspects[edit]

Deconvolution maps to division in the Fourier co-domain. This allows deconvolution to be easily applied with experimental data that are subject to a Fourier transform. An example is NMR spectroscopy where the data are recorded in the time domain, but analyzed in the frequency domain. Division of the time-domain data by an exponential function has the effect of reducing the width of Lorenzian lines in the frequency domain.

Absorption spectra[edit]

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Deconvolution has been applied extensively to absorption spectra.^[7] The Van Cittert algorithm (in German) may be used.^[8]

See also[edit]

References[edit]

1. ^O'Haver T. 'Intro to Signal Processing - Deconvolution'. University of Maryland at College Park. Retrieved 2007-08-15.
2. ^Wiener N (1964). Extrapolation, Interpolation, and Smoothing of Stationary Time Series. Cambridge, Mass: MIT Press. ISBN0-262-73005-7.
3. ^ ^abCheng PC (2006). 'The Contrast Formation in Optical Microscopy'. Handbook of Biological Confocal Microscopy (Pawley JB, ed.) (3rd ed.). Berlin: Springer. pp. 189–90. ISBN0-387-25921-X.
4. ^Nasse M. J., Woehl J. C. (2010). 'Realistic modeling of the illumination point spread function in confocal scanning optical microscopy'. J. Opt. Soc. Am. A. 27 (2): 295–302. doi:10.1364/JOSAA.27.000295. PMID20126241.
5. ^Ahi, Kiarash; Anwar, Mehdi (May 26, 2016). 'Developing terahertz imaging equation and enhancement of the resolution of terahertz images using deconvolution'. Proc. SPIE 9856, Terahertz Physics, Devices, and Systems X: Advanced Applications in Industry and Defense, 98560N. doi:10.1117/12.2228680.
6. ^Sung, Shijun (2013). Terahertz Imaging and Remote Sensing Design for Applications in Medical Imaging. UCLA Electronic Theses and Dissertations.
7. ^Blass, W.E.; Halsey, G.W. (1981). Deconvolution of Absorption Spectra. Academic Press. ISBN0121046508.
8. ^Wu, Chengqi; Aissaoui, Idriss; Jacquey, Serge (1994). 'Algebraic analysis of the Van Cittert iterative method of deconvolution with a general relaxation factor'. J. Opt. Soc. Am. A. 11 (11): 2804–2808. doi:10.1364/JOSAA.11.002804.

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